The article deals with with the possibility of researching and mapping female painters’ self-portraits with the key of queer interpretation and with the accent on women’s and queer writings. The research of female self-portraits from this perspective addresses the new approach to modern art history, and the possibilities of construction/conceptualization/readings/reflection of gender identity of the artist herself as author and observer of the painting. Theoretical analysis of self-portraits and individual works from the opus of Nadežda Petrović, Danica Jovanović, Milena Pavlović Barili, Nasta Rojc, Beta Vukanović and Natalija Cvetković was conducted.